Figure 5

RSPO1, FOXL2 and c-Kit immuno-detection on goat ovaries. a) 40 dpc ovaries. b) 50 dpc ovaries. Scale bars: 100 μm. The fluorescent staining is presented alone (left column) or with a DAPI blue nuclear-specific counterstaining (medium and right columns). The right column corresponds to a 5.0 enlargement of the red rectangle depicted on the medium column. At 40 dpc, RSPO1 is detected in the cortical area (co) of the ovaries where most of c-Kit positive germ cells lies. At this stage both somatic and germ cells are stained (arrows show 2 germinal cells). By contrast, FOXL2 positive cells are in the sub-cortical area (sc) of these early developing ovaries. At 50 dpc, RSPO1 is detected mainly around the c-Kit positive germ cells easily recognizable by their large and round nuclei (arrows). At this stage, FOXL2 positive somatic cells are located in the two ovarian compartments, cortex and medulla. m = mesonephros; the dotted line delimits both areas (co and sc).