Figure 3
From: R-spondin1 and FOXL2act into two distinct cellular types during goat ovarian differentiation
RT-PCR expression analyses of R-spondin genes in XX PIS-/- sex-reversed goat gonads. Expression profiles of FOXL2 and the four R-spondin genes were determined at 3 developmental stages (36, 40 and 50 dpc) in XX sex-reversed gonads (XX Males) in comparison with normal males and females ones. GAPDH was used as control. The name of the amplified gene and the number of PCR cycles done is given on the left margin. Samples are from individual fetuses (identified by their number) or from a pool of 3 gonads (N = 3). L: 100 bp DNA ladder molecular weight marker (Bioline).