Figure 2

Expression of G-CSF and its receptor on the RNA level. G-CSF receptor (A) and its ligand G-CSF (B) are present throughout murine embryonic development, which is demonstrated by RT-PCR. RT-PCR products of the following stages were loaded on the gels: E11, E12, E14, E16, E18 and P2. As positive controls for G-CSF and G-CSF receptor sequence-verified cDNA clones were used. The negative control consisted of water. RNA was prepared out of E11 and E12 whole embryonic mouse heads, for the other stages mouse brain was taken. Primers are located in the 3' part of the open reading frames with product lengths of 235 bp for G-CSF receptor and 360 bp for G-CSF. (Pos., positive PCR control (G-CSF or G-CSF receptor, respectively; neg., negative control)