Figure 2

Teneurin-1 is expressed in interconnected regions of the brain and is complementary to teneurin-2 expression. Immunostaining of teneurin-1 and teneurin-2 in E17 brain. Green, teneurin-1; red, teneurin-2, blue (Hoechst), nuclei. In the olfactory bulb (A, B) the teneurin-1 antibody labels the mitral cell layer (mcl) and the glomerular layer. The external plexiform layer (epl) is positive for teneurin-2. In the temporal retina (C, D) subsets of neurons within the ganglion cell layer (gcl) and inner portion of the internal nuclear layer (inl) are positive for teneurin-1, as are laminae 2 and 5 within the inner plexiform layer (ipl). Teneurin-2 immunoreactivity is concentrated in laminae 1 and 3 of the ipl. The nucleus rotundus (E, ROT) is intensely labelled with teneurin-1 antibody, but not with anti-teneurin-2 (F). In the optic tectum (G, H) large neurons in the stratum griseum centrale (SGC) are positive for teneurin-1, but not teneurin-2. In the hindbrain (I, J), the nucleus laminaris (La) and nucleus magnocellularis (MCC) are labelled with the teneurin-1 antibody. Teneurin-1 and teneurin-2 immunoreactivity are found in distinctive dendritic fields within the La. In the cerebellum (K, L) teneurin-1 is found in Purkinje cells (Pcl) and in the molecular layer (ml) adjacent to the external granular layer (egl). In contrast, the anti-teneurin-2 labels the internal portion of the ml.