Figure 4

Morphological rescue of some moe^- embryonic defects by injection of the epb4.1l5 constructs. (A) In wild-type embryos at 60 hpf, brain ventricles are visible, and the RPE is uniform (inset). (B) In moe^- embryos, brain ventricles are reduced in size or absent, pericaridal edema is pronounced, and the RPE is patchy (inset). (C) In moe^- embryos injected with epb4.1l5^{short+long_PBD}mRNA, brain ventricles are reduced in size or absent, pericaridal edema is pronounced, but the RPE is normal (inset). (D) moe^- embryos injected with epb4.1l5^{long+short_PBD}mRNA, brain ventricles are absent or absent and pericardial edema is pronounced, and RPE defects are milder than those in uninjected moe^- embryos. (E) A magnified view of the RPE of a 60 hpf wild-type embryo shows that it is uniform and the cells are confluent. (F) In a wild-type retina at 4 dpf, GFP⁺ rods localize next to the RPE and lamination is apparent. In 60 hpf moe^- mutants, the integrity of the RPE varies from mild (G), to moderate (I), to severe (H). However, GFP⁺ rods are mislocalized in all moe^- mutants 4 dpf (H, J, L). The integrity of the RPE is improved and nearly normal in a 60 hpf moe^- mutant injected with epb4.1l5^{long+short_PBD}mRNA (M) and most GFP⁺ rods are adjacent to the RPE (N). (O) A wild-type embryo injected with epb4.1l5^{long+short_PBD}showing brain ventricles that are reduced or absent. (P) At 30 hpf Epb4.1l5^{long+short_PBD} is cortically localized, upper inset is a 2× magnification of Epb4.1l5^{long+short_PBD} localization. Scale bars, 10 μm (F), 50 μm (lower insets in F).