Figure 6

Cholesterol and isoprenoids rescue statin-induced PGC survival and migration defects. (A) Average PGC survival in tissue treated with the indicated combinations of cholesterol (1× concentration), GGOH (pooled data from 10 uM and 20 uM treatments), FOH (10 uM) and atorvastatin (10 uM). " * " = groups that differed from the atorvastatin group (F < 0.05 Analysis of Variance from Walpole and Meyer with Fishers least significance post test). (B) The percentage of slices in each treatment group that had two PGC clusters after culture. "*" = groups that differed significantly from the atorvastatin group (exact binomial test P-value < 0.05) (C) Control slice that exhibited robust migration (two distinct PGC clusters) and survival (108%). (D) Atorvastatin treated slice with scattered PGCs and reduced survival (56%). (E) Slice treated with cholesterol, FOH and atorvastatin with excellent survival (100%), but poor migration. (F) Slice treated with atorvastatin, cholesterol and GGOH with robust migration and survival (133%). Scale bar is 100 μm. Error bars are s.e.m. n = number of slices.