Table 3 Oligonucleotides used for PCR
Oligo | Sequence |
|---|---|
C-term TAP F | ATGGAAAAGAGAAGATGGAAAAAG |
C-term TAP R | GGTTGACTTCCCCGC |
FLAG-GFP F | ATGGATTACAAGGACGATGACGATAAGATGAG |
FLAG-GFP R | CAAAGCTTGTGGGCTTTTGTATAG |
N-term TAP F | ATGGCAGGCCTTGCGC |
N-term TAP R | AAGTGCCCCGGAGGATGAGATTTTCT |
R6K F | CCTTAGAGGCTATTTAAGTTGCTG |
R6K R | GTACTAAGCTCTCATGTTTCAC |
RT screen F | ACGTTAACCGGGCTGCAT |
RT screen R | GCCGTCAATAAGTTCTGTCAA |
unc-119 F | CAAATCCGTGACCTCGACAC |
unc-119 R | CACAGTTGTTTCTCGAATTTGG |