Figure 2

Genetic and molecular characterization of elp-1 . (A) The physical map of elp-1 is drawn in the reverse orientation. Two alternatively spliced transcripts, elp-1a and elp-1b, are shown with exons as solid boxes and introns as lines. We confirmed the identity of the PCR products by a diagnostic restriction digest with EcoR1 and BglII (Panel A, right). (B) Genetic structures of the elp-1::gfp expression constructs pKA99-1, pKA99-2 and pKA99-3. Exons are indicated by black boxes. The proteins encoded by these constructs are indicated as P_elp-1::GFP, ELP- 1Δ::NLS::GFP, and ELP-1::GFP, respectively in the Text. (C) Amino acid sequence of ELP-1a showing a potential alternative start site for elp-1(ok347) (black arrow), exon 5 aa sequence (magenta), HELP motif (green) and the beginning of the WD domain (red arrow). (D) PCR reaction with a forward primer in the deletion site and a reverse primer in exon 11. There is no detectable wild-type copy of the elp-1 gene in the deletion strain as evidenced by the absence of the 1105 bp band. (E) The elp-1(ok347) strain retains two mRNAs of approximately 2200 and 2100 bp.