Figure 4

Unique genes that are expressed in glial precursors NAE. (A) shows the extent of overlap for expressed genes of three distinct types of cell populations, NAE, unsorted SM and astrocyte precursor hAPC. A total of 458 transcripts are expressed at ≥ 100 as detected by the array uniquely to NAE (detailed gene list can be found in Additional file 2). A selected group of genes (B) are validated by RT-PCR (C). In (B), the numbers in columns NAE, SM and APC represent the expression levels of these samples. The units are arbitrary as the signals are obtained by comparing with the background signals of the array chip. The numbers in columns NAE/SM and NAE/APC represent the ratios of the expression levels of the listed genes. The RT-PCR results (C) validate that all 10 genes listed in (B) have higher expression for NAE than for SM or APC, while the expression for the internal control GAPDH is similar across all three samples.