Figure 2

A2B5+/PSA-NCAM- MACS-sorted cells show properties of glial precursors. Immunocytochemistry analysis was done for the double MACS-enriched A2B5+/PSA-NCAM- (NAE) cells. Approximately 87% of NAE cells are A2B5+ (B) and ~9% are PSA-NCAM+ (D), They have high immunoreactivity to several glial (GFAP; C) and glial precursor markers (NG2 and PDGFRα; F and G). In contrast, lower percentage of cells show markers specific for the neuronal lineage, such as PSA-NCAM (D) and TuJ1 (βIII tubulin, E).