Figure 2
From: Regionalisation of the mouse visceral endoderm as the blastocyst transforms into the egg cylinder
Lack of growth restriction between the embryonic and extra-embryonic regions of the VE. Examples of labelled conceptuses recovered after microinjection of single cells at the surface of the ICM at the blastocyst stage. They are shown as a fluorescent projection of a confocal z series merged with a transmitted light section, at the early egg cylinder (EEC) stage (A, C, F), E5.5 (B, D, G) and E6.5 (E, H). For each stage, the number of labelled conceptuses considered (n) and the percentage of those with colonisation of the embryonic region only (Emb, A-B), the extra-embryonic region only (Xemb, C-E) or both (Xemb+Emb, F-H) is indicated. Arrowheads point to the embryonic/extra-embryonic junction. Conceptuses are oriented with the anterior pole on the left, whenever it can be identified. Scale bars 20 μm.