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Table 4 Details of the primers used for real-time quantitative PCR analysis and in situ hybridization

From: Alterations in transcript abundance of bovine oocytes recovered at growth and dominance phases of the first follicular wave

Gene name

Gene bank accession Number

Primer sequences

Annealing temperature (°C)

Product size (bp)

BMP15a

AY304484

F: 5'- CTGACGCAAGTGGACACCCTA -3'

60

396

  

R: 5'- GACACACGAAGCGGAGTCGTA -3'

  

PTTG1a

NM_004219

F: 5'- GAAGAGCACCAGATTGCGC -3'

55

204

  

R: 5'- GTCACAGCAAACAGGTGGCA -3'

  

MSX1a

NM_174798

F: 5'- AAGGTATCCACAGTCCCCAGC -3'

55

180

  

R: 5'- TCTGCCTCTCCTGCAAAGTTC -3'

  

PPa

AF170490

F: 5'- GCTGCATCCTACTTGTCGGAA-3'

55

194

  

R: 5'- TTCCAAACTACAACCGCCTTG -3'

  

S100A10a

NM174681

F: 5'- GGATTTCTGAGCATATGGGACC -3'

55

131

  

R: 5'- GAGCAAGAGGATGCAAGCAATA -3'

  

ANXA2a

NM_174716

F: 5'- CGTGCTCCAGCTAACAGTTCT-3'

55

139

  

R: 5'- GGAAAGCCAGGTAATGCGTA-3'

  

CKS2a

NM_001827

F: 5'- CGACGACGAGGAGTTCGAGTA-3'

55

122

  

R: 5'- CCTGACTCTGCTGAACACCAAG-3'

  

RPL24a

NM_174455

F: 5'- CCGTGCAGTCAAATTCCAAA-3'

55

242

  

R: 5'- CAACTCGAGGAGCAGAAACCTT-3

  

FL396a

AY308068

F: 5'- CGCCAACACGTCTTATACCAAC-3'

55

201

  

R: 5'- CTGCGAAGAGGCTTCCAATTAG-3'

  

CCNB1a

L26548

F: 5'- CGATATGGTGCACTTTCCTCC-3'

55

145

  

R: 5'- TGACCACATTCTTTGCCAGG-3'

  

GAPDHa

BC102589

F: 5'-ACCCAGAAGACTGTGGATGG-3'

60

247

  

R: 5'-ACGCCTGCTTCACCACCTTC-3'

  

MSX1b

NM_174798

F:5'-AGAAGCAGTACCTGTCCATCG

55

382

  

R: 5'-GGCCTTCTATGTCAGGTGGTA

  

MSX1c

NM_174798

T7-F:5'- GTAATACGACTCACTATAGGGAGAAGCAGTACCTGTCCATCG

55

382

  

R: 5'-GGCCTTCTATGTCAGGTGGTA

  

MSX1d

NM_174798

SP6-R:5'- GATTTAGGTGACACTATAGAAGGCCTTCTATGTCAGGTGGTA

55

382

  

F:5'-AGAAGCAGTACCTGTCCATCG

  
  1. a The primer used for real-time PCR, b The primer used for DNA template amplification, c The forward primer coupled with T7 promoter (underlined) used for sense probe synthesis, d The reverse primer coupled with SP6 promoter (underlined) used for antisense probe synthesis.