In situ electroporation | Retroviral transduction | |||
---|---|---|---|---|
Asset | Limitation | Asset | Limitation | |
7–7.5 dpc: Mesodermal/pre-hematopoietic precursors | - Transient - Layer specific delivery - Spatial control of delivery | - Reduced number of precursors available for transduction | - Causes extensive cell death - Refractory to transduction ? | |
Application | Only possible method | Unsuitable at this development stage | ||
From 7.5–8 dpc: Hematopoietic precursors | - Transient - Layer specific delivery - Spatial control of delivery | - Only a fraction of the YS blood islands is targeted | - Easy - All hematopoietic precursors may be transduced | - Does not allow layer specific targeting |
Application | -Transient transgene delivery | - Sustained expression of the transgene | ||
- Possible layer specific delivery. | - Possible delivery of ShRNA | |||
- Possible delivery of SiRNA or proteins |