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Figure 2 | BMC Developmental Biology

Figure 2

From: Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction

Figure 2

Plasmid injection protocol. Abbreviations: am: amnion; EPC: Ectoplacental cone; YSC: YS cavity OB-EB stage embryos are dissected from the decidua (A). The Reichert membrane is removed, while the EPC is kept in place (B). The plasmid solution is injected into the YSC, from the node region through the amnion (C). Plasmid filling of the YSC is visualised with fast green (D). Prior to pulse application, the electrodes are positioned parallel to the prospective blood islands ring (E). After electroporation, the YS is dissected from the embryo and placed in organ culture (D). Scale bar: 100 μm.

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BMC Developmental Biology

ISSN: 1471-213X

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