Figure 4

foxi1 and vent but not dlx3b are direct targets of Bmp-signaling during gastrulation. (A, B, E, F, I, J) Expression of foxi1, dlx3b and vent is expanded after Bmp2a protein injection at late blastula stages in comparison to wild-type embryos. (C, D, G, H, K, L) Pharmacological inhibition of protein synthesis with cycloheximide (CHX) at late blastula stages blocks all indications of foxi1, dlx3b and vent expression (C, G, K), whereas foxi1 and vent but not dlx3b expression is restored in CHX-treated embryos after Bmp2a protein injection (D, H, L). Lateral views at the end of gastrulation with dorsal to the right and anterior towards the top (note that CHX treatment blocks epiboly movements). Scale bar: 90 μm.