Figure 1

Spatial reconstruction of gene expression using laser microdissection. Above left – DAPI stain. Circled area indicates approximate size and cell number in each LMD sample. Left – nine sections were used for LMD that spanned the anterior, middle, and posterior bulb. Middle – four LMD samples were isolated from each section, sampling the DM, DL, VM, and VL aspects of the nascent EPL/GL. Right – RNA from each LMD sample was amplified and applied to microarrays. Bottom – data from the 36 arrays was synthesized to generate a three-dimensional statistical estimate of gene expression within the bulb for each gene on the array.