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Table 1 Array-based expression ratios of lineage-specific markers in the OCT4, NANOG, and SOX2 knock-downs

From: Silencing of core transcription factors in human EC cells highlights the importance of autocrine FGF signaling for self-renewal

Lineage

Ratio

Ratio

Ratio

/GENE

OCT4 kd

NANOG kd

SOX2 kd

endoderm

   

AFP

n/a

n/a

n/a

FGF8

n/a

n/a

n/a

GATA4

n/a

n/a

n/a

GCK

n/a

n/a

n/a

HNF4A

n/a

n/a

n/a

ONECUT1

n/a

n/a

n/a

ectoderm

   

FGF5

n/a

n/a

n/a

HOXB1

n/a

n/a

n/a

LHX5

2.9**

1.0

1.7*

NEUROD1

n/a

n/a

n/a

NOG

n/a

n/a

n/a

OLIG2

n/a

n/a

n/a

OTX1

n/a

n/a

n/a

PAX6

n/a

n/a

n/a

SOX1

n/a

n/a

n/a

mesoderm

   

ACTA2

22.6**

n/a

22.6**

ACTC

9.2**

n/a

9.2**

BMP4

5.9**

1.1

5.3**

CD34

n/a

n/a

n/a

COL1A1

3.2**

n/a

4.4**

COL2A1

8.4**

n/a

8.0**

HAND1

9.1**

3.8**

8.1**

MSX1

4.9**

n/a

4.8**

MYOD1

n/a

n/a

n/a

RUNX1

5.3**

n/a

5.5**

T

n/a

n/a

n/a

trophoblast

   

CDX2

n/a

n/a

n/a

CSH1

n/a

n/a

n/a

HCG

n/a

n/a

n/a

KRT7

n/a

n/a

n/a

prim. endoderm

   

GATA6

1.4

n/a

1.3

  1. With the exception of LHX5, BMP4, and HAND1, none of the genes was expressed at detectable levels in mock-treated hEC cell cultures. We did not investigate whether T was transiently up-regulated during cellular differentiation. For the calculation of expression ratios reference intensities were arbitrarily set to the level of threshold expression. n/a denotes insignificant expression also in the RNAi samples. * and ** indicate statistical significance of the expression changes (P < 0.05 and < 0.01, respectively).
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BMC Developmental Biology

ISSN: 1471-213X

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