Figure 4

The putative dorsal glial restricted progenitor population can generate macroglial subtypes in mass culture. (A,C,D) Putative glial restricted progenitor cells generate GalC+ cells (A) GFAP+ cells (C) but do not generate neurons (D) after 6 days of exposure to the appropriate differentiation conditions (see Results). (B) After 4 days of growth in the pro-oligodendrocyte condition, O4+ cells were readily identifiable. (E,F) Exposure of the putative glial restricted progenitor population to BMP-4 is insufficient to result in detection of the known astrocyte marker GFAP until 10 days (E), but does induce the Astrocyte Precursor Cell marker, CD44, after 6 days (F). DAPI, blue nuclear stain (D,F). Scale bars, 100 μm.