Figure 3

Outline of the isolation procedure used to characterize the putative glial restricted progenitor population. A2B5+/PSA-NCAM- cells were selected by MACS resulting in a heterogeneous mixture of cells. For mass culture studies (A) and clonal analysis (B), cells were maintained in culture for two cell passages to select for proliferative cells and remove the A2B5+ neuronal population (C). The resultant putative glial restricted progenitor population was then plated at mass culture or clonal density and exposed to differentiating conditions including a pro-oligodendrocytic condition, a pro-astrocytic condition, or a pro-neuronal condition (see Materials and Methods). Alternatively, the heterogeneous mixture of cells obtained from the MACS selection was plated at clonal density, and resultant clones were selectively passaged and split into the differentiation conditions (D).