Figure 11

Dorsal glial restricted progenitors and explant derived dorsal glial restricted progenitors produce compact myelin, in addition to the ability of both ventral and dorsal glial restricted progenitors to make astrocytes in vivo. (A-C') EM images from the contralateral hemisphere of the transplanted shiverer forebrains showed a lack of dense, compacted myelin, consistent with the shiverer mutant phenotype, on longitudinally sectioned (A) and cross-sectioned (A') neuronal fibers. The dorsal glial restricted progenitor isolated from the E15 dorsal telencephalon and transplanted into the P18 shiverer forebrain is capable of myelin formation as seen in longitudinally sectioned (B) and cross-sectioned (B') neuronal fibers. Transplantation of the dorsal glial restricted progenitor cell derived from two day in vitro grown E13 dorsal telencephalic explants into the P18 shiverer mutant forebrain produces compacted myelin as seen in longitudinally sectioned (C) and cross-sectioned (C') neuronal fibers. (D-F) hPAP+ dorsal glial restricted progenitors transplanted into the forebrains of P0 rat pups generate hPAP+/GFAP+ cells after 10 days, as well as Olig2+ oligodendroglial cells (G-I). DAPI, blue nuclear stain (F). Scale bars for A-C' as indicated, scale bars for D-I, 100 μm.