Figure 10

A glial restricted progenitor population cell can be isolated from the E15 ventral telencephalon. (A,B,D) Putative glial restricted progenitor cells sharing the similar antigenic profile of the dorsal glial restricted progenitor population were isolated from the E15 ventral telencephalon, consisting of the AEP and MGE. This cell population generated GalC+ cells (A) GFAP+ cells (B) but not neurons (D) in mass culture. (C) Putative glial restricted progenitor cells do not make A2B5+/GFAP+ type-2 astrocytes in response to CNTF. (E-G), To distinguish between APC/OPC presence and glial restricted progenitor presence, ventral putative glial restricted progenitor cells were grown at clonal density and generated GalC+ cells (E) GFAP+ cells (F) but not neurons (G) when examined at the clonal level. (H-J) Split clones of ventral putative glial restricted progenitor founder cells generated GalC+ cells (H) GFAP+ cells (I) but not neurons (J). DAPI, blue nuclear stain, (A,C-J). Scale bars, 100 μm.