Figure 8
SDF-1 signaling activates downstream genes. (a-d) Illustrates data from Affymetrix rat genome RG-230v2 microarrays analysis; every data point represents the average of 3 independent hybridizations (biological replicates) per experimental condition. (a) Illustrates the change in the mean expression level of all hybridized transcripts between control and SDF – treatment. (b, c) Illustrates transcripts that showed a near 2-fold (37%) or greater than 2-fold (12%) up regulation in SDF-1 treatment (b), and a near 2-fold (72%) down regulation in AMD3100 treatment (c) compared to controls. (d) Cluster analysis shows genes of biological significance being grouped together (e) qRT-PCR analysis to validate the gene expression of Cx 43 and Sfrp1.