Figure 1

Generation of TgDHCR7 mice. Panel A shows a cartoon of the transgene construct used for microinjection. The human Apo E promoter containing non-coding exon 1 (E1), intron 1 and part of non-coding exon 2 (E2) was fused to a N-terminal 3HA tagged human DHCR7 cDNA containing intron 5, followed by a human Apo E poly A addition site (pA), and a liver enhancer element. Panel B shows the results of the Southern blot analysis of Bam HI-digested DNA from the three TgDHCR7 (Tg) founder mice (lane 3, 4 and 5). Lane 1 is λ-Hind III DNA marker and lane 2 contains DNA from a Tg negative control animal. The arrowhead points to the expected size bands for TgDHCR7 (2.3 kb). Additional bands (asterisks) indicate the integrated transgene that has undergone some deletions or rearrangements.