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Figure 2 | BMC Developmental Biology

Figure 2

From: Replacement of connexin43 by connexin26 in transgenic mice leads to dysfunctional reproductive organs and slowed ventricular conduction in the heart

Figure 2

A. RT-PCR analyses of Cx43 and Cx26 mRNA expression in brain, heart, liver and testis. Fragments: Cx43: 293 bp, Cx26: 364 bp. RNA from HM-1 embryonic stem cells and distilled water served as controls. Equal loading was demonstrated by actin amplicons (243 bp). B. Western blot analyses of Cx43 and Cx26 protein expression in heart lysates. Cx43 protein was not detected in homozygous Cx43KI26 animals (lane 3). Cx26 was not found in wild-type heart (43/43), whereas it was expressed in heterozygous (43/26) mice and at nearly the double amount in homozygous Cx43KI26 animals. Loading of equal amounts of homogenates was verified by re-immunolabeling of the same membrane with monoclonal anti-tubulin.

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BMC Developmental Biology

ISSN: 1471-213X

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