Table 2 Primer pairs designed for semiquantitative RT-PCR. The number of PCR cycles is indicated for ACH-3P cells and first trimester trophoblasts.

RPL30

NM_000989

for: CCT AAG GCA GGA AGA TGG TG

rev: CAG TCT GTT CTG GCA TGC TT

ACH-3P: 20

FT: 25

HLAG

NM_002127

for: AGG AGA CAC GGA ACA CCA AG

rev: CCT CCA GGT AGG CTC TCC TT

ACH-3P: 25

FT: 27

ITGA1

NM_181501

for: GTC CAG TTG GGA GAG GTG AA

rev: TGA ATG CCT CCT TTC TTG CT

ACH-3P: 26

ITGA5

NM_002205

for: TCC TGG AGT CCT CAC TGT CC

rev: TCC TTG GCA GTA ACC CTG TC

ACH-3P: 23

ITGA6

NM_000210

for: GGC GGT GTT ATG TCC TGA GT

rev: CAT GCT CAG TCT CTC CAC CA

ACH-3P: 25

MMP2

NM_004530

for: TTG GCA GTG CAA TAC CTG AA

rev: GAG CAA AGG CAT CAT CCA CT

ACH-3P: 20

MMP9

NM_004994

for: GAG ACC GGT GAG CTG GAT AG

rev: CAC CAA ACT GGA TGA CGA TG

ACH-3P: 35

MMP14

NM_004995

for: AAG GCA CAC TTG CTC CTG TT

rev: CAC TGG TGA GAC AGG CTT GA

ACH-3P: 23

FT: 25

MMP15

NM_002428

for: GGC CGA CAT CAT GGT ACT CT

rev: GTC AAC GTC CTT CCA CTG GT

ACH-3P: 24

FT: 26

EDN1

NM_001955

for: CTC GTC CCT GAT GGA TAA A

rev: TCC TGC TTG GCA AAA ATT C

ACH-3P: 35

FT: 35

EDNRA

NM_001957

for: ATC ACC GTC CTC AAC CTC TG

rev: CCA ATT CCC TGA ACA CGA CT

ACH-3P: 35

FT: 35

EDNRB

NM_000115

for: ATC TGC GAA TCT GCT TGC TT

rev: AGC CAC CAA TCT TTT GCT GT

ACH-3P: 33

FT: 35

β-hCG

NM_000737

for: GTC AAC ACC ACC ATC TGT GC

rev: GGC CTT TGA GGA AGA GGA GT

ACH-3P: 24

IGF1

NM000618

for: TGG ATG CTC TTC AGT TCG TG

rev: CCT GCA CTC CCT CTA CTT GC

ACH-3P: 35

FT: 35

IGF2

NM_000612

for: GGT GCT TCT CAC CTT CTT GG

rev: GGG GTA TCT GGG GAA GTT GT

ACH-3P: 25

FT: 25

IGF1R

NM_000875

for: GGA TGC GGT GTC CAA TAA CT

rev: TGG CAG CAC TCA TTG TTC TC

ACH-3P: 29

FT: 32

IGF2R

NM_00876

for: TGA GTA TGC CTG CCA CAG AG

rev: TCA AGG TGA GGT CTC CAT CC

ACH-3P: 26

FT: 29

INSR

NM_000208

for: GAG TCC TCG TTT AGG AAG ACG

rev: AAG TGT TGG GGA AAG CTG CCA

ACH-3P: 31

FT: 33

TNFA

NM_000594

for: GAC AAG CCT GTA GCC CAT GT

rev: TTG ATG GCA GAG AGG AGG TT

ACH-3P: 33

FT: 33

TNFR1

NM_001065

for: ACC AAG TGC CAC AAA GGA AC

rev: CTG CAA TTG AAG CAC TGG AA

ACH-3P: 25

FT: 27

TNFR2

NM_001165

for: CCA AGT GGT TTC CAA GGT GT

rev: CAC GGC AGC ATT AAT CAC AG

ACH-3P: 29

FT: 31