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Figure 1 | BMC Developmental Biology

Figure 1

From: The first trimester human trophoblast cell line ACH-3P: A novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations – TNF-α stimulates MMP15 expression

Figure 1

Morphology and trophoblast marker expression of parental fusion partners and hybrid cells. The photomicrographs representative shows cultured first trimester trophoblasts (FT) 48 hours after isolation, ACH-3P hybrid cells and AC1-1 choriocarcinoma cells, both in the 6th passage (20 × magnification) (A). IHC staining for cytokeratin-7 and vimentin of ACH-3P (B). Representative zymography of cell culture supernatants from FT, ACH-3P and AC1-1 after 48 h culture indicating presence of MMP9 and MMP2 (C). Representative FACS analysis for HLA-G (thick line) on viable ACH-3P cells (D). The result of the control antibody is shown (thin line) as overlay.

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BMC Developmental Biology

ISSN: 1471-213X

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