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Table 2 H. bacteriophora and C. elegans RNAi Phenotypes

From: Postembryonic RNAi in Heterorhabditis bacteriophora: a nematode insect parasite and host for insect pathogenic symbionts

 

Trial

Nematode Gene (Phenotype)

1 %Pen.(n)A

2 %Pen.(n)

3 %Pen.(n)

4 %Pen.(n)

5 %Pen.(n)

6 %Pen.(n)

H. bacteriophora B

(-)water (Ste)

0 (49)

0 (17)

0 (20)

0 (39)

0 (40)

0 (30)

Hba-cct-2

100 (34)

100 (36)

100 (16)

96 (24)

94 (64)

100 (34)

Hba-daf-21

100 (36)

100 (19)

100 (30)

92 (24)

82 (34)

87 (47)

Hba-icd-1

100 (31)

100 (38)

87 (15)

100 (15)

98 (40)

98 (40)

Hba-nol-5

95 (39)

100 (32)

100 (41)

93 (30)

57 (28)

72 (32)

Hba-W01G7.3

95 (39)

100 (34)

95 (39)

100 (33)

83 (47)

86 (49)

Hba-rack-1

31 (39)

82 (22)

28 (25)

61 (28)

10 (39)

33 (33)

Hba-arf-1

50 (32)

67 (15)

47 (15)

ndC

nd

nd

Hba-ben-1

0 (48)

38 (21)

11 (36)

0 (45)

0 (42)

0 (33)

Hba-nhr-47

nd

12 (17)

3 (36)

nd

nd

nd

Hba-mrp-4

0 (57)

10 (21)

0 (26)

nd

nd

nd

C. elegans D

Cel-cct-2 (Pvl)

83 (86)

71(97)

53 (92)

nd

nd

nd

(Ste)

100 (86)

100 (97)

100 (92)

nd

nd

nd

Cel-icd-1 (Pvl)

81 (118)

86 (114)

nd

nd

nd

nd

(Ste/Stp)

100(118)

100 (114)

nd

nd

nd

nd

Cel-W01G7.3 (Pvl)

10 (94)

9 (64)

12 (69)

nd

nd

nd

(Ste)

100 (94)

100 (64)

<100 (>50)

nd

nd

nd

(Egl, Stp)

nd

nd

few

nd

nd

nd

Cel-nol-5 (Pvl)

68 (94)

64 (98)

nd

nd

nd

nd

(Ste)

100 (94)

100 (98)

nd

nd

nd

nd

Cel-rack-1(Pvl)

19 (66)

4 (80)

3 (72)

nd

nd

nd

(Ste)

100 (66)

<100(>50)

<100(>50)

nd

nd

nd

(Egl, Stp)

0

Few

few

nd

nd

nd

Cel-ben-1

wtE (>50)

wt (>50)

wt (>50)

nd

nd

nd

  1. A Percent penetrance (% Pen.), number of worms examined (n).
  2. B Number of sterile adult female or hermaphrodites with defective gonads from RNAi by soaking, see methods for details.
  3. C Not determined
  4. D Postembryonic phenotypes from RNAi by feeding, see methods for details.
  5. E Indistinguishable from wt.