Figure 3

Pld localizes to cytoplasmic vesicles during cellularization. A. Pld localization in heat-fixed y¹w^67c23control embryos using an affinity-purified anti-Pld antisera (sagittal image generated at or near the embryonic equator). a, apical; n, nuclei; b, basal. B. Affinity-purified pre-immune serum does not generate a visible signal. C. Pld localization in transgenic lines overexpressing Pld as a maternal transcript using NGT-GAL4. D. Pld localization in transgenic lines expressing Pld RNAi. E, F. Pld does not co-localize with p120-marked Golgi vesicles (red) in wild-type (sagittal section) or Pld; NGT40+A embryos (surface view). G-J. Cellularizing embryos immunostained with anti-Pld antisera (green), monoclonal anti-actin (red), and Propidium iodide (blue). Pld in early cellularizing embryos (G, H) co-localizes with actin at sites where adjacent cells are forming junctions (inset). Later in cellularization (I-J), Pld does not localize to sites at which actin tracks have been assembled (inset).