Figure 2
From: The Hlx homeobox transcription factor is required early in enteric nervous system development
Ontogeny of ENS development in Hlx wild-type and mutant embryos. Sections from littermate Hlx+/+ (panels A, C, and E) and Hlx-/- (panels B, D, and F) embryos at E12.5 (panels A and B), E14.5 (panels C and D), and E16.5 (panels E and F) were stained for PGP9.5 as described in Methods with the primary antibody at a titer of 1:800. Sections were chosen to identify complementary regions of the GI tract. Examples of PGP9.5+ cells are indicated by blue arrowheads. Black bars, 100 μm. By E12.5, wild-type intestine is well populated with PGP9.5+ cells. Clustering of PGP9.5+ cells is evident thereafter. However, in Hlx-/- embryos, only a few PGP9.5+ cells are seen at any timepoint.