Figure 2

Embryos homozygous for targeted deletion of cubilin do not express cubilin transcripts. Shown are RT-PCR analyses of the expression of cubilin transcripts in RNA from heterozygous (+/-) and null (-/-) 8.5 dpc embryos. In A, a primer pair corresponding to a 5' region of the cubilin transcript was used to assess cubilin transcript expression. In B, a primer pair corresponding to a 3' region of the cubilin transcript was used to assess cubilin transcript expression.