Figure 4From: MATER protein expression and intracellular localization throughout folliculogenesis and preimplantation embryo development in the bovineExpression of bovine MATER in oocytes and preimplantation embryos. Developmental stages are immature oocytes (IO), in vitro matured oocytes (MO), in vitro cultured zygote (1C), 2-, 4-, 5- to 8- cell embryos, morula (mo), expanded blastocysts (EB) and hatched blastocysts (HB). (A) Quantification of the messenger RNA using real-time PCR. Relative level to immature oocytes is shown (mean ± SEM). In late stage embryos, transcript level is indicated but too low to be seen on the histogram. Different superscripts indicate a significant difference (P ≤ 0.05). (B) Detection of the protein by Western blot; α-TUBULIN is shown as a positive control.Back to article page