Skip to main content

Table 2 Percentage of labeled epithelium following transfection, electroporation or adenoviral infection. The percentage of epithelial cells labeled represents the average counts from 100 cells over five fields from three different buds.

From: In vitroculture of embryonic mouse intestinal epithelium: cell differentiation and introduction of reporter genes

 

% explants with any expression

% explants with epithelial expression

% epithelial cells

GeneJuice

75 (3/4)

0 (0/4)

0

Electroporation

73.8 (31/42)

11.9 (5/42)

0.9 ± 0.3

AdLacZ - Dispase

100 (24/24)

25 (6/24)

2.3 ± 1.3

AdLacZ + Dispase

100 (25/25)

68 (17/25)

29.1 ± 1.1***

  1. *** p < 0.001. One-way ANOVA (non-parametric). SEM given for % epithelial cells labeled.