Table 1 Antibodies
Experiment | Primary | Supplier | Secondary | Supplier |
|---|---|---|---|---|
AdLacZ | mouse monoclonal anti-E-cadherin 1/50 | BD Transduction Laboratories | horse anti-mouse Texas Red conjugated IgG 1/100 | Vector |
|  | rabbit polyclonal anti-β-galactosidase 1/300 | ICN | goat anti-rabbit fluorescein isothiocyanate (FITC) conjugated IgG 1/100 | Vector |
GeneJuice | mouse monoclonal anti-E-cadherin 1/50 | BD Transduction Laboratories | horse anti-mouse FITC conjugated IgG 1/100 | Vector |
| Â | rabbit polyclonal anti-DsRed 1/3000 | Clontech | Swine anti-rabbit tetramethylrhodamine isomer R (TRITC) conjugated IgG 1/100 | DAKO |
Small Intestine characterisation | Rat monoclonal anti-E-cadherin 1/100 | Zymed | goat anti-rat Texas Red conjugated IgG 1/100 | Vector |
| Â | rat monoclonal anti-Troma-1 (cytokeratin 8) 1/50 | DSHB, University of Iowa | goat anti-rat Texas Red conjugated IgG 1/100 | Vector |
|  | mouse monoclonal anti-α smooth muscle actin 1/100 | Sigma | horse anti-mouse FITC conjugated IgG 1/100 | Vector |
| Â | mouse monoclonal anti-sucrase-isomaltase 1/200 | Gift (Dr Hans-Peter Hauri) | horse anti-mouse FITC conjugated IgG 1/100 | Vector |
| Â | mouse monoclonal anti-cdx2 1/100 | BioGenex | horse anti-mouse FITC conjugated IgG 1/100 | Vector |
| Â | rabbit polyclonal anti-lysozyme 1/100 | DAKO | goat anti-rabbit FITC conjugated IgG 1/100 | Vector |
| Â | rabbit polyclonal anti-chromogranin A 1/100 | DAKO | goat anti-rabbit FITC conjugated IgG 1/100 | Vector |
|  | goat polyclonal anti-HNF-4α 1/100 | Santa Cruz | rabbit anti-goat FITC conjugated IgG 1/100 | Vector |