Figure 4

Gene delivery to embryonic intestine. (A) and (B) DsRed expression 24 hours after GeneJuice transfection (DsRed is here coloured green to permit direct comparison with the images in the rest of the figure). There is no expression of DsRed in the epithelium (E-cadherin, red). (C) and (D) Electroporation of pcDNA3nucGFP2 into the intestine. GFP is visualised predominantly in the mesenchyme after 24 hours culture. (E) and (F) Infection of intestine explants with Ad-CMV-LacZ adenoviral vector. E-cadherin is shown in red and β-galactosidase in green 48 hours after infection. (G) and (H) Pretreatment of intestinal explants with dispase substantially enhances adenoviral-mediated gene delivery to the epithelium. E-cadherin is shown in red and β-galactosidase in green. Representative cultures are shown for each regime. Scale bars = 100 μm A, C-E, G; 20 μm B, F, H.