Table 1 Numbers of copies of integrated transgenes This was estimated from the average of the ratios between fluorescence intensities using primers for human PAX6 and 0.5 × the fluorescence intensities using primers for mouse Pax3 or Pax6 in qPCR reactions on a constant amount of DNA from each of a series of embryos. Each qPCR reaction was repeated three times on each animal.

From: Functional conservation of Pax6 regulatory elements in humans and mice demonstrated with a novel transgenic reporter mouse