Table 3 Expression of hESC specific markers in pooled hESC sample as detected by Illumina bead array The expression of previously identified hESC markers was examined in all hESC samples (the values displayed represent the expression level of pooled H1, H7 and H9). Most of the genes were also identified using Illumina bead array in all 8 hESC populations in this study (1*), the gene CER1 was detected in all except one duplicate of H9 (2*), Nanog was not detected in all populations (3*) and Sox2, Lin41, NR6A1 and FoxD3 were not detected in the array although they were present in the chips for hybridization (4*).

NM_003641.1

IFITM1

8844

1*

NM_020997.2

LEFTB

6579.6

1*

NM_024674.3

LIN28

3944.1

1*

NM_175849.1

DNMT3B

3391

1*

NM_003212.1

TDGF1

3169

1*

NM_001769.2

CD9

2930.8

1*

NM_000165.2

GJA1

2404.4

1*

NM_021195.2

CLDN6

2247.2

1*

NM_004360.2

CDH1

1972.9

1*

NM_021127.1

PMAIP1

1601.5

1*

NM_032805.1

ZNF206

1504.5

1*

NM_003577.1

UTF1

1444.1

1*

XM_050625.2

SFRP2

1353.9

1*

NM_006548.3

IMP-2

1206

1*

NM_152312.2

GYLTL1B

1066.6

1*

NM_015973.2

GAL

1043.3

1*

NM_003240.2

EBAF

944.1

1*

NM_054023.2

SCGB3A2

890.5

1*

NM_020990.2

CKMT1

742.4

1*

NM_033668.1

ITGB1

694.7

1*

NM_003744.3

NUMB

618.3

1*

NM_007015.1

LECT1

597.4

1*

NM_021912.2

GABRB3

482.7

1*

NM_006729.2

DIAPH2

467.1

1*

NM_000222.1

KIT

188.9

1*

NM_005454.1

CER1

151.5

2*

NM_024865.1

NANOG

56.9

3*

NM_002701.1

POU5F1

694.4

1*

NM_003106

SOX2

ND

4*

NM_006458.2

LIN41

ND

4*

NM_001489.3

NR6A1

ND

4*

NM_012183

FOXD3

ND

4*