Table 2 Distribution of genes with expression levels <50 and >50–10,000 as detected by Illumina bead array in 8 hESC populations All human ESC samples were hybridized in one experiment and the relative detection levels of genes were binned to obtain a global overview of transcription, approximately 8, 000 genes (~50%) were greater than 100 arbitrary units. The numbers are similar to results obtained by other large scale analysis such as MPSS.
Abundance (relative detection levels) | H9 | H9 on human feeders | I6 | BG01 | BG02 | BG03 | BG01V | Pooled (H1, H7, H9) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. | % | No. | % | No. | % | No. | % | No. | % | No. | % | No. | % | No. | % | |
<50 | 2,909 | 21.2 | 6,067 | 38.4 | 3,559 | 25.3 | 4,528 | 30.7 | 4,256 | 30.5 | 4,706 | 32.2 | 419 | 4.3 | 5,803 | 34.4 |
>50 | 10,817 | 78.8 | 9,747 | 61.6 | 10,484 | 74.7 | 10,232 | 69.3 | 9,694 | 69.5 | 9,915 | 67.8 | 9,438 | 95.7 | 11,085 | 65.6 |
>100 | 8,126 | 59.2 | 7,539 | 47.7 | 7,409 | 52.8 | 7,703 | 52.2 | 7,496 | 53.7 | 7,430 | 50.8 | 7,230 | 73.3 | 8,217 | 48.7 |
>500 | 3,077 | 22.4 | 2,947 | 18.6 | 2,626 | 18.7 | 3,065 | 20.8 | 2,950 | 21.1 | 2,852 | 19.5 | 2,851 | 28.9 | 2,941 | 17.4 |
>1000 | 1,629 | 11.9 | 1,625 | 10.3 | 1,490 | 10.6 | 1,638 | 11.1 | 1,589 | 11.4 | 1,517 | 10.4 | 1,566 | 15.9 | 1,554 | 9.2 |
>5000 | 248 | 1.8 | 257 | 1.6 | 256 | 1.8 | 256 | 1.7 | 263 | 1.9 | 262 | 1.8 | 275 | 2.8 | 251 | 1.5 |
>10000 | 90 | 0.7 | 94 | 0.6 | 112 | 0.8 | 92 | 0.6 | 100 | 0.7 | 107 | 0.7 | 101 | 1.0 | 94 | 0.6 |
Total No. of genes detected at >0.99 confidence | 13,726 | 15,814 | 14,043 | 14,760 | 13,950 | 14,621 | 9,857 | 16,888 | ||||||||