Table 1 Correlation coefficients of paired samples in this bead array In order to test the reproducibility and reliability of the bead array, duplicate samples of hESC lines H9, I6, and EC line NTera2 and human fibroblast feeders (HS27) were run at the same time and correlation coefficients (R2) of duplicates were generated using the entire data of all genes with expression level >0 (§), or genes with detection confidence >0.99 (*), or genes with detection confidence >0.99 and expression level > 100 arbitrary units (#). Note that the correlation coefficients are in the range of 0.9382–0.9761 and the number of genes was in the range of 10,000–14,000.
Duplicate Samples | No. of all genes (expr.>0)§ | R2of all genes (expr. >0) | No. of genes (>0.99)* | R2 of genes (>0.99)* | No. (>0.99, level>100)# |
|---|---|---|---|---|---|
H9 | 18,899 | 0.8681 | 13,672 | 0.9708 | 7,408 |
I6 | 19,139 | 0.8663 | 12,570 | 0.9761 | 6,826 |
NTera2 | 19,162 | 0.8741 | 14,036 | 0.9382 | 7,147 |
Feeder | 18,157 | 0.8724 | 10,606 | 0.9751 | 7,021 |