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Figure 5 | BMC Developmental Biology

Figure 5

From: Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S. Federal government approved hESC lines

Figure 5

Male and female hESC lines can be distinguished by genes identified by bead array. Five potential genes RPS4Y, RPS4Y2, EIF1AY, VCY, and AMELY are located in the Y chromosome. By comparing the expression level of these genes in all hESC lines, we have found that 3 out of 5 were specifically expressed in male hESC lines I6, BG01 and BG02 (A) and this was verified by RT-PCR in male line BG01 and female line BG03 (B). G3PDH was used as an internal control. *: represents the gene expression level is detected at <0.99 confidence.

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BMC Developmental Biology

ISSN: 1471-213X

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