Figure 7From: Aberrant stalk development and breakdown of tip dominance in Dictyosteliumcell lines with RNAi-silenced expression of calcineurin BExpression of ecmB mRNA in AX2 and 01–5 cells. Total RNA was prepared from cells developed on HABP filters for the times (in hours) indicated, separated on 0.9% agarose/6.6% formaldehyde gels, blotted onto nylon membranes and hybridized with a DIG-labelled probe for ecmB. A probe for actin15 was used as loading control (signals in the lower part). Note that expression of actin15 decreases during development.Back to article page