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Table 1 Summary list of antibody verification by western blot.

From: Development of antibodies to human embryonic stem cell antigens

Antibody

Sample used for analysis

Mol. Wt. (KD)

Gt × hBrachyury

mouse ES-derived EB lysate

48

Ms × hDPPA5

N/A

N/A

Gt × hGATA6

Caco2 cell lysate

65

Gt × hNanog

NTERA-2 cell lysate

33

Gt × hOct 3/4

NTERA-2 cell lysate

39

Gt × hPDX1

beta-TC 6 cell lysate

32

Gt × hSOX17

mouse ES-derived EB lysate

45

Ms × hCD9

PBMC

25

Rt × hGATA-1

N/A

N/A

Ms × hE-Cadherin

MCF-7 cell lysate

97

Ms × hPODXL

MCF-7 cell lysate

57

Ms × hSOX2

NTERA-2 cell lysate

36

  1. N/A: 1. DPPA5 is still being subcloned. Only Elisa verification is available.
  2. 2. The clone for GATA-1 (MAB1779) does not work for Western blot application but is useful for IHC, The clone picked for Western blot analysis does not work for IHC (MAB17791, see data in http://www.rndsystems.com).
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BMC Developmental Biology

ISSN: 1471-213X

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