Figure 7
From: The roles of Bcl-xL in modulating apoptosis during development of Xenopus laevis
Maternal and zygotic XR11 RNA expression and protein levels. A. Over-expression of XR11 at the RNA level. RT-PCR of the constitutively expressed elongation factor 1-alpha (EF1α) was conducted in parallel as a control. This experiment was conducted once. B. XR11 protein levels in pre- and post-MBT embryos were demonstrated by Western blot analysis. Because the XR11 protein is membrane-bound, it is difficult to separate it from yolk, which is abundant in embryos. The presence of yolk during electrophoresis results in wavy bands in the gel. This experiment was repeated once.