Figure 3
From: The roles of Bcl-xL in modulating apoptosis during development of Xenopus laevis
Metamorphosis of XR11 transgenic tadpoles and responses to exogenous thyroid hormone (T 3 ). A , B. An XR11 transgenic froglet undergoing metamorphosis. C-J. Responses of one-week old (approximately stage 45) transgenic tadpoles to thyroid hormone (T3). XR11 transgenic tadpoles (G-J;) display the same gross responses to 5 days exposure to as do GFP transgenics (C-F). C, E, G and Iare live tadpoles, whereas D, F, Hand Jare fixed tadpoles stained with Alcian blue to reveal the skeleton and the gill apparatus. K-R. The apoptotic response of one-week old transgenic tadpoles (approximately stage 45) to T3 was assessed in cross-sections through the velum. DAPI staining (K, M, Oand Q) reveals nuclei, whereas the TUNEL assay (L, N, Pand R) distinguishes apoptotic nuclei. The velum of the GFP transgenic tadpole (M, N) has begun dissociating in response to T3 treatment. S. Demonstration of XR11 over-expression in tadpoles by immunoprecipitation. This experiment was repeated once.