Figure 5
From: Gdt2 regulates the transition of Dictyosteliumcells from growth to differentiation
Phenotype of the gdt 2- mutant. (A) Colony phenotype. 500 cells of wild type and either g14 (the knockout clone) or IR32 (the original REMI mutant) were spotted on the same SM agar plate and left to grow for 5 days. Both REMI and knockout clones show very narrow or absent clearing zones. (B) Overexpression of discoidin I by gdt2- mutant at low cell densities. Ax2 and g14 cells were grown in bacterial suspension and harvested at a density of 1 × 106 cells/ml. Discoidin I was detected by Western blot analysis with an anti-discoidin I antibody.