Figure 1

Identification of the gdt 2 gene. (A) Schematic map of the gdt2 gene. The coding sequence is shown as open boxes, the site of REMI insertion is indicated with an arrow. The site of the Blasticidin insertion in the knockout construct is shown with triangle. Sequences used as probes for Southern and Northern blots are schematically shown below. (B) Southern blot analysis of one of the gdt2 knockout clones, g14, and wild type Ax2 cells. Genomic DNA was digested with BclI and hybridized with the probe shown in (A). (C) Predicted amino acid sequence of GDT2. The GDT2 protein is composed of 1637 amino acids. The signal sequence (AA 1-21) is boxed and the putative transmembrane domains (AA 129-145, 167-184 and 897-921) are underlined. The putative kinase domain (AA 1284-1535) is shown in bold.