Figure 5From: IfkA, a presumptive eIF2α kinase of Dictyostelium, is required for proper timing of aggregation and regulation of mound sizeDetection of acaA and carA mRNAs in BS153 and the parental strain, Ax4. Total RNA was isolated from growing cells (0) and at the times indicated (in hours) after the onset of development. RT-PCR was carried out using oligonucleotides specific for either acaA mRNA or carA mRNA. H7 specific oligonucleotides were used as an internal control (H7 is a constitutively expressed gene).Back to article page