Figure 5
From: NF-κB DNA-binding activity in embryos responding to a teratogen, cyclophosphamide
NF-κB complex formation in the embryonic brain and liver.A: Nuclear extracts from the brain (lanes 1–4) and liver (lanes 5–8) of E13 and E 14, control and exposed to 40 mg/kg CP. 1 – E13, control; 2 – E13, CP; 3 – E14, control; 4 – E14, CP; 5 – E13, control; 6 – E13, CP; 7 – E14, control; 8 – E14, CP; 9 – no sample.B: Supershift analysis of -κB-binding proteins presented in nuclear extract from the brain of E13 demonstrating p65/p50 heterodimers (top band) and p50/p50 homodimers (bottom band). Specificity of binding was determined by competition with 100-fold excess of unlabeled NF-κB oligonucleotide or 100-fold excess of OCT oligonucleotide.