Figure 4

Autofluorescence is reduced following treatment with copper sulfate. (A-L) Single confocal sections of the prepharyngeal region of planarians excited with 405 nm (A, B, and C), 488 nm (D, E, and F), 561 nm (G, H, and I), and 633 nm (J, K, and L) laser lines. Planarians exhibit significant autofluorescence at several wavelengths (A, D, G, and J) that increases following a 16 hour incubation at 56°C (B, E, H, and K). Increased autofluorescence is significantly reduced at all wavelengths following a 1 hour incubation in copper sulfate solution (C, F, I, and L). (M and N) Maximum intensity projection of the dorsal posterior of a planarian following FISH for Smed-EGFR-5 before (M) or after (N) copper sulfate quenching of autofluorescence. Arrows indicate the same position in M and in N. Scale bar: 100 μm.