Figure 3

Addition of Triton X-100 to blocking and wash buffers improves signal-to-noise ratio for peroxidase-conjugated anti-hapten antibodies. Planarians were incubated, as indicated, with DIG-, DNP-, or FAM-labeled anti-sense RNA probes to Smed-PKD1 and then blocked in 5% horse serum with 0.5% RWBR, incubated in anti-hapten antibody, and washed in Tris-sodium chloride buffer containing either 0.05% Tween (A, B, and C), 0.3% Triton X-100 (D, E, and F), or 0.05% Tween and 0.3% Triton (G, H, and I). Images are maximum intensity projections of planarian heads imaged using identical settings and false colored in ImageJ using the fire look up table. Scale bar: 100 μm.