Figure 1
From: Histone modifications in the male germ line of Drosophilaa
Spermatogonia and somatic testis cells. (a-c) H3K9me2; D. melanogaster, spermatogonia (Stg). Label in cytoplasm (Cy) and in heterochromatin (N: nucleus). (d-f) H4K20me1; D. melanogaster, small diploid cells of the testis envelope (dc) and one cell of low polyteny (pc). The label appears in a dotted pattern in the euchromatin (EU) while heterochromatin (HET) remains unlabeled. (g-i) H3K9me2; D. hydei, diploid cells of the testis envelope. Heterochromatin (chromocenter) and a few spots in the nucleus react with this antibody. (j-l) H3K9me3; D. melanogaster, polytene cell with labeled heterochromatin (HET). Also here, few spots appear in euchromatin. Cytoplasm and nucleus display background only. (a, d, g, j) DAPI; (b, e, h, k) antibody reaction; (c, f, i, l) merged pictures of DAPI and antibody.